Rearrange: Solute Volume = 300 μL / 10 = 30 μLĪnswer: Perform a 1:10 dilution that makes at least 30 μL (e.g.Plug values in: (300 μL) / Solute Volume = 10. Choose step DFs: Need a total dilution factor of 1000.Example: Make only 300 μL of a 1:1000 dilution, assuming the smallest volume you can pipette is 2 μL.Use the formula: Final DF = DF1 * DF2 * DF3 etc., to choose your step dilutions such that their product is the final dilution. If the dilution factor is larger than the final volume needed, or the amount of stock is too small to be pipetted, one or more intermediary dilutions may be required. Answer: Place 1.2 μL of the stock solution into 300 μL – 1.2 μL = 298.8 μL diluent.Rearrange: Solute Volume = 300 μL / 250 = 1.2 μL.Plug values in: (300 μL) / Solute Volume = 250.Formula: Final Volume / Solute Volume = DF.
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